| Expression pattern: |
DN |
| Associated gene: |
KDM6B, ELF3, H3K27me3, NLRP3, cleaved Caspase-1, GSDMD-N, IL-1beta, IL-18 |
| Associated microRNA: |
miR-342-3p |
| Biological function: |
iPSC-MSC-EV-carried circ-CCND1 inhibits DSS-induced pyroptosis of colonic epithelial cells, increases cell viability, and reduces inflammatory cytokines and pyroptosis markers. |
| Molecular mechanism: |
EV-delivered circ-CCND1 binds KDM6B, reduces KDM6B enrichment at the ELF3 promoter, increases H3K27me3 at the ELF3 promoter, suppresses ELF3, increases miR-342-3p, and miR-342-3p targets KDM6B to form a feedback loop that inhibits pyroptosis. |
| Biological pathway or process: |
pyroptosis (inhibits); inflammation (inhibits); mRNA stability (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); ChIP / ChIP-seq; Luciferase Reporter Assay; CCK8; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential novel therapeutic strategy for UC; no human tissue validation was performed. |
| Description: |
In this in vitro UC model, circ-CCND1 expression decreased after DSS treatment and was delivered into colonic epithelial cells by iPSC-MSC-derived extracellular vesicles. EV-carried circ-CCND1 binds KDM6B and modulates the KDM6B/ELF3/miR-342-3p feedback axis and H3K27me3-related histone methylation, thereby suppressing inflammatory pyroptosis. |
| Confidence score: |
0.6649 |