| Expression pattern: |
UP |
| Associated gene: |
ROCK2 |
| Associated microRNA: |
miR-302b-3p |
| Biological function: |
circ_HN1 acts as an oncogenic circRNA in gastric cancer, promoting tumor growth, cell proliferation, colony formation, cell cycle progression, migration and invasion, while inhibiting apoptosis. |
| Molecular mechanism: |
circ_HN1 functions as a ceRNA by sponging miR-302b-3p, thereby upregulating ROCK2 expression to facilitate gastric cancer progression. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Colony Formation Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model |
| Clinical significance: |
circ_HN1 is a potential target for GC therapy; high circ_HN1 expression might be related to GC advancement. |
| Description: |
circ_HN1 is upregulated in gastric cancer tissues and cells and functions as an oncogenic circRNA. It promotes GC progression by sponging miR-302b-3p and upregulating ROCK2, thereby enhancing proliferation, colony formation, cell cycle progression, migration, invasion and tumor growth while suppressing apoptosis. The study suggests circ_HN1 as a potential therapeutic target for gastric cancer. |
| Confidence score: |
0.7052 |