| Expression pattern: |
UP |
| Associated gene: |
PRG4, FOXO1, SLC7A11, GPX4 |
| Associated microRNA: |
miR-568 |
| Biological function: |
circRNA_0030042 knockdown promoted viability and proliferation and repressed apoptosis and ferroptosis in H2O2-induced AC16 cells. |
| Molecular mechanism: |
ceRNA mechanism in which hsa-circRNA_0030042 binds miR-568 and positively regulates PRG4 via miR-568. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); ferroptosis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence) |
| Clinical significance: |
hsa-circ_0030042 expression showed diagnostic potential for heart failure with AUC 0.8872. |
| Description: |
Human hsa_circ_0030042 is up-regulated in peripheral blood from heart failure patients and in H2O2-induced AC16 cardiomyocytes. Its knockdown protects the in vitro heart failure model by improving viability/proliferation and suppressing apoptosis and ferroptosis through the miR-568/PRG4 regulatory axis, and it may have diagnostic value for heart failure. |
| Confidence score: |
0.8429 |