| Expression pattern: |
DN |
| Associated gene: |
FMRP, TFAP2A, Vimentin |
| Associated microRNA: |
miR-576-5p |
| Biological function: |
Inhibits CRC cell proliferation, migration, EMT, invasion and metastasis; overexpression suppresses tumor growth in vivo. |
| Molecular mechanism: |
circ_0009022 acts as a ceRNA for miR-576-5p to regulate TFAP2A and binds the RBP FMRP, reducing Vimentin expression and inhibiting EMT. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Co-IP |
| Clinical significance: |
circ_0009022 is negatively associated with tumor growth, invasion, metastasis and outcomes, and may serve as a potential biomarker and therapeutic target for CRC. |
| Description: |
hsa_circ_0009022/circ_0009022 is down-regulated in CRC tissues and cell lines and acts as a tumor-suppressive circRNA. It inhibits proliferation, migration, metastasis and EMT by sponging miR-576-5p to regulate TFAP2A and by binding FMRP to reduce Vimentin-related EMT activity. The study suggests circ_0009022 may be a potential biomarker and therapeutic target for CRC. |
| Confidence score: |
0.8388 |