| Expression pattern: |
UP |
| Associated gene: |
PRIM2 |
| Associated microRNA: |
miR-378b |
| Biological function: |
circ_0078710 promotes malignant phenotypes of HCC cells and attenuates curcumin-mediated anti-tumor effects; circ_0078710 silencing suppresses proliferation, migration, invasion and tumor growth, and promotes apoptosis under curcumin treatment. |
| Molecular mechanism: |
circ_0078710 acts as a molecular sponge for miR-378b and positively regulates PRIM2 expression, thereby mediating the anti-tumor effects of curcumin in HCC cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; EdU Staining; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
- |
| Description: |
circ_0078710 is up-regulated in HCC cell lines and is reduced by curcumin treatment. Functionally, circ_0078710 silencing enhances curcumin-mediated suppression of proliferation, migration, invasion and xenograft tumor growth, while promoting apoptosis. Mechanistically, circ_0078710 sponges miR-378b to positively regulate PRIM2 in the circ_0078710/miR-378b/PRIM2 axis. |
| Confidence score: |
0.7289 |