| Expression pattern: |
UP |
| Associated gene: |
IGF2BP1, IRAK1, IRAK1 3′UTR |
| Associated microRNA: |
- |
| Biological function: |
Promotes colon cancer cell proliferation, migration, invasion, metastasis and tumor growth; inhibits M1 macrophage polarization and increases M2 macrophage polarization. |
| Molecular mechanism: |
Tumor-derived exosomal circ_0020095 is transferred to M0 macrophages and competitively binds IGF2BP1, reducing IGF2BP1 binding to IRAK1 3′UTR and inhibiting IRAK1 expression, thereby suppressing M1 macrophage polarization. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); macrophage polarization (other); immune regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; EdU Staining; Wound Healing Assay; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Bioinformatics Analysis; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis |
| Clinical significance: |
High circ_0020095 expression was associated with lower 5-survival rates and correlated with tumor size, distant metastasis and TNM stage of CC patients. |
| Description: |
circ_0020095 is up-regulated in colon cancer tissues, cells and CC cell-derived exosomes, and high expression is associated with poorer survival and more aggressive clinicopathological features. Functionally, tumor-derived exosomal circ_0020095 promotes CC proliferation, migration, invasion, metastasis and tumor growth by suppressing M1 macrophage polarization. Mechanistically, circ_0020095 competitively binds IGF2BP1 and blocks IGF2BP1 binding to IRAK1 3′UTR, reducing IRAK1 expression and thereby regulating the IGF2BP1/IRAK1 axis. |
| Confidence score: |
0.8349 |