| Expression pattern: |
UN |
| Associated gene: |
PKR |
| Associated microRNA: |
- |
| Biological function: |
AAV-delivered circCAMSAP1(2,3) dampens PKR activation, reduces proinflammatory cytokines, decreases Abeta plaques and Tau pathology, alleviates gliosis and neuronal loss, and improves spatial learning and memory in AD mouse models. |
| Molecular mechanism: |
circCAMSAP1(2,3) is a human ds-cRNA containing three short duplex regions; as a ds-cRNA aptamer it suppresses PKR activation, thereby reducing neuroinflammation and AD-related pathological phenotypes. |
| Biological pathway or process: |
immune regulation (inhibits); inflammation (inhibits); proliferation (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
B
|
| Validation methods: |
Northern Blot; RT-qPCR; IF (Immunofluorescence); ELISA; Western Blot; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
Human circCAMSAP1(2,3) was used as an independent ds-cRNA aptamer to test whether therapeutic effects were not sequence-specific to circPOLR2A. AAV delivery of circCAMSAP1(2,3) reduced PKR activation and neuroinflammation, alleviated AD-related pathology, and improved learning and memory in 5xFAD and PS19 mouse models. |
| Confidence score: |
0.4636 |