circRNA basic information
circBase ID: hsa_circ_0006156
Name: hsa_circ_FNDC3B
Synonym: circRNA fibronectin type III domain containing 3B / circ-FNDC3B
Host Gene: FNDC3B
Genomic location(hg19): chr3:171965322-171969331:+
Genomic location(hg38): chr3:172247532-172251541:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005350
MONDO name: abdominal aortic aneurysm
Disease details: abdominal aortic aneurysm / AAA
Disease DO ID:
 7693
Disease MeSH ID:
 D017544
Disease NCIt ID:
 -
Disease ICD11 ID:
 1154633768
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

AAA aorta tissues; normal aorta tissues; aortic specimens
Cell lines:

Human aortic VSMCs (#PCS-100-012); primary VSMCs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: ADAM10, Ago2
Associated microRNA: miR-143-3p
Biological function: Knockdown of circ-FNDC3B alleviated Ang-II-induced VSMC injury by improving viability and proliferation and reducing apoptosis, inflammation, and oxidative stress.
Molecular mechanism: circ-FNDC3B acts as a competing endogenous RNA for miR-143-3p to regulate ADAM10 expression.
Biological pathway or process:

ceRNA regulation (promotes); apoptosis (promotes); proliferation (inhibits); inflammation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot
Clinical significance:

circ-FNDC3B expression was positively correlated with the diameter of AAA in patients.
Description:

This study identifies human circ-FNDC3B (hsa_circ_0006156) as an up-regulated circRNA in AAA aorta tissues and Ang-II-treated VSMCs. circ-FNDC3B promotes Ang-II-induced VSMC injury by acting as a ceRNA for miR-143-3p, thereby increasing ADAM10 expression and enhancing apoptosis, inflammation, oxidative stress, and impaired VSMC growth. Its expression also correlates positively with AAA diameter, suggesting potential relevance to AAA development.
Confidence score:

0.7795
Other information
Title:

Identification of Circ-FNDC3B, an Overexpressed circRNA in Abdominal Aortic Aneurysm, as a Regulator of Vascular Smooth Muscle Cells.

Journal: International heart journal
Published: 2021
PubMed ID: 34789642
Study type:

combined biological and clinical study
Data availability: Please contact the correspondence author for the data request; supplemental files at https://doi.org/10.1536/ihj.21-186
Code availability: -