| Expression pattern: |
UP |
| Associated gene: |
Bcl-2, Atg12 |
| Associated microRNA: |
miR-140-3p |
| Biological function: |
circ_0000231 promotes CRC cell proliferation, migration, invasion, tumorigenesis, and autophagy; downregulation of circ_0000231 suppresses proliferation and migratory abilities and promotes G0/G1 phase arrest. |
| Molecular mechanism: |
circ_0000231 acts as an RNA sponge for miR-140-3p, upregulates Bcl-2, and enhances autophagy; Bcl-2 interacts with Atg12 to regulate autophagy. |
| Biological pathway or process: |
autophagy (promotes); proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); ceRNA regulation (other); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); Co-IP; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0000231 might serve as a diagnostic and prognostic indicator and a novel molecular target for CRC therapy; high expression is associated with poor prognosis. |
| Description: |
circ_0000231 is upregulated in colorectal cancer tissues and high expression is associated with poor prognosis. Functionally, circ_0000231 promotes CRC cell proliferation, migration, invasion, xenograft growth, and autophagy. Mechanistically, it sponges miR-140-3p to regulate Bcl-2, with Bcl-2-associated autophagy involving Atg12. |
| Confidence score: |
0.8051 |