circRNA basic information
circBase ID: hsa_circ_0065149
Name: hsa_circ_SETD2
Synonym: -
Host Gene: SETD2
Genomic location(hg19): chr3:47098310-47103836:-
Genomic location(hg38): chr3:47056820-47062346:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005385
MONDO name: vascular disorder
Disease details: vascular remodeling
Disease DO ID:
 178
Disease MeSH ID:
 D014652
Disease NCIt ID:
 C35117
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

human atherosclerotic arteries; human carotid plaques; mouse common carotid arteries; vascular tissues
Cell lines:

HASMCs / human aortic smooth muscle cells
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: p-414aa, HuR, C-FOS mRNA, SETD2 pre-mRNA, upstream and downstream flanking intronic regions of circSETD2 (14,15)
Associated microRNA: -
Biological function: Inhibits VSMC phenotypic switching, suppresses VSMC proliferation and migration, promotes differentiation of PDGF-BB-treated VSMCs through its encoded protein p-414aa, and inhibits neointimal hyperplasia and vascular remodeling.
Molecular mechanism: circSETD2(14,15) encodes p-414aa in an IRES-dependent manner; p-414aa binds HuR, disrupts HuR binding to C-FOS mRNA, accelerates C-FOS mRNA degradation, lowers C-FOS protein levels, and inhibits VSMC proliferation and vascular remodeling. HuR also promotes circSETD2(14,15) circularization and stability by binding flanking intronic regions and the mature circRNA sequence.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); mRNA stability (inhibits); other pathway/process (inhibits)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Co-IP; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IF (Immunofluorescence); Western Blot; Bioinformatics Analysis; RNA-seq
Clinical significance:

CircSETD2(14,15) and p-414aa may serve as potential therapeutic targets for vascular remodeling diseases.
Description:

circSETD2(14,15), also identified as hsa_circ_0065149, is reduced in human atherosclerotic arteries and PDGF-BB-induced proliferative HASMCs. It encodes the hidden protein p-414aa, which binds HuR and reduces HuR-mediated stabilization of C-FOS mRNA, thereby suppressing VSMC proliferation, migration, phenotypic switching, neointimal hyperplasia, and vascular remodeling.
Confidence score:

0.8213
Other information
Title:

A Novel Hidden Protein p-414aa Encoded by circSETD2(14,15) Inhibits Vascular Remodeling.

Journal: Circulation
Published: 2025
PubMed ID: 40099364
Study type:

combined biological and bioinformatics study
Data availability: GSE152280; GSE77278; GSE77279; GSE43292; GSE281385; Supplemental Material; Table S1; Table S2; Table S3; Table S4; Table S5; Table S6; Table S7
Code availability: -