| Expression pattern: |
UP |
| Associated gene: |
FZD4, EIF4A3, Ago2 |
| Associated microRNA: |
miR-361-3p |
| Biological function: |
promotes OS cell proliferation and migration, promotes EMT, promotes tumor growth and lung metastasis, and reduces sensitivity to cisplatin |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-361-3p to increase FZD4 expression and activate FZD4/Wnt/beta-catenin signaling; circPRKAR1B biogenesis is promoted by EIF4A3 binding to downstream flanking sequences. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); EMT (promotes); metastasis (promotes); apoptosis (inhibits); Wnt/beta-catenin (promotes); chemoresistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Sanger Sequencing; divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circPRKAR1B is up-regulated in osteosarcoma and mainly localized in the cytoplasm. It promotes proliferation, migration, EMT, tumor growth and lung metastasis by sponging miR-361-3p to increase FZD4 and activate Wnt/beta-catenin signaling; EIF4A3 facilitates its biogenesis. circPRKAR1B overexpression decreases sensitivity to cisplatin (CDDP), suggesting a role in chemoresistance. |
| Confidence score: |
0.848 |