| Expression pattern: |
DN |
| Associated gene: |
TET1, AGO2, 5-hydroxymethylcytosine (5hmC) |
| Associated microRNA: |
miR-191 |
| Biological function: |
Suppresses HCC cell proliferation, migration, invasion and immune evasion; inhibits tumor growth and pulmonary metastasis in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-191 to protect/upregulate TET1 (mainly at protein level), affecting DNA demethylation (5hmC). |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); immune regulation (other); ceRNA regulation (promotes); m6A modification (not specified) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; CCK8; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); circRIP; RNA Pull-Down; Luciferase Reporter Assay; RNA-seq; Bioinformatics Analysis |
| Clinical significance: |
Low circTRIM33-12 expression correlates with malignant characteristics and is an independent risk factor for overall survival (OS) and recurrence-free survival (RFS) in HCC patients after surgery. |
| Description: |
circTRIM33-12 is down-regulated in HCC and acts as a tumor suppressor. It sponges miR-191 to relieve repression of TET1 (increasing TET1 protein), influencing 5hmC/DNA demethylation and reducing proliferation, migration/invasion, metastasis and immune evasion; low circTRIM33-12 predicts worse prognosis and recurrence risk. |
| Confidence score: |
0.8295 |