| Expression pattern: |
UP |
| Associated gene: |
ITGA11, EIF4A3 |
| Associated microRNA: |
miR-29b-3p |
| Biological function: |
Promotes GC cell proliferation, migration, invasion, and EMT; induces cell cycle progression (knockdown causes G0/G1 arrest). |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-29b-3p to increase ITGA11 expression, thereby promoting EMT; EIF4A3 binds flanking regions and inhibits circRNA_100290 formation. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Western Blot; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; Bioinformatics Analysis |
| Clinical significance: |
High circRNA_100290 expression was closely related to Borrmann’s type, lymph node metastasis and tumour-node-metastasis stage; proposed as a biomarker/target. |
| Description: |
circRNA_100290 is upregulated in gastric cancer tissues and cell lines and promotes proliferation, migration, invasion, cell-cycle progression, and EMT. It acts as a ceRNA by sponging miR-29b-3p to upregulate ITGA11, while EIF4A3 binds flanking regions to inhibit circRNA_100290 formation. |
| Confidence score: |
0.705 |