| Expression pattern: |
UP |
| Associated gene: |
IL6, JAK2, STAT3 |
| Associated microRNA: |
miR-495 |
| Biological function: |
Promotes CRC cell proliferation, migration, invasion and tumor growth; acts as an oncogenic circRNA associated with poor prognosis. |
| Molecular mechanism: |
circ_0000372 functions as a competitive endogenous RNA by sponging miR-495, thereby elevating IL6 expression and activating the JAK2/STAT3 signaling pathway. |
| Biological pathway or process: |
JAK/STAT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High expression is associated with poor prognosis; circ_0000372 could be used as a new prognostic biomarker and therapeutic target for CRC. |
| Description: |
circ_0000372 is an APLP2-derived circRNA that is upregulated in colorectal cancer tissues and cell lines, and high expression predicts poorer overall survival. Functionally, it promotes CRC proliferation, migration, invasion and xenograft tumor growth. Mechanistically, cytoplasmic circ_0000372 sponges miR-495 to increase IL6 expression and enhance JAK2/STAT3 signaling. |
| Confidence score: |
0.835 |