| Expression pattern: |
UP |
| Associated gene: |
MAP3K9, p38, ERK1/2, Ago2 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
Circ_DLG1 promotes ESCC cell proliferation, cell cycle progression, viability, migration, invasion, metastasis, and in vivo tumor growth; circ_DLG1 knockdown inhibits these malignant phenotypes. |
| Molecular mechanism: |
Circ_DLG1 acts as a ceRNA/miRNA sponge for miR-338-3p, thereby upregulating MAP3K9 and activating the p38/ERK pathway in ESCC. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes); MAPK (promotes); ERK (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; Cell Cycle Assay; MTT; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_DLG1 expression was linked to relatively poor overall survival of ESCC patients and higher expression was observed in stage III + IV tumor tissues compared with stage I + II. |
| Description: |
Circ_DLG1 is up-regulated in ESCC tissues, plasma, and cell lines, and high expression is associated with poorer overall survival. Functionally, circ_DLG1 promotes ESCC proliferation, migration, invasion, metastasis, and xenograft tumor growth. Mechanistically, it sponges miR-338-3p to increase MAP3K9 expression and activate the p38/ERK pathway. |
| Confidence score: |
0.8198 |