| Expression pattern: |
UP |
| Associated gene: |
FOXA1, CEACAM6, BRD4 |
| Associated microRNA: |
- |
| Biological function: |
Promotes colorectal cancer cell proliferation, migration, invasion, tumor growth and liver metastasis, and inhibits apoptosis. |
| Molecular mechanism: |
circDIAPH1 recruits transcription factor FOXA1 to the CEACAM6 promoter to initiate CEACAM6 expression; BRD4 regulates circDIAPH1 expression. |
| Biological pathway or process: |
apoptosis (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; ROC Analysis; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; RNA-seq; Luciferase Reporter Assay; ChIP / ChIP-seq; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot |
| Clinical significance: |
Potential biomarker for CRC liver metastasis with the highest AUC value among six LM-DE-circRNAs. |
| Description: |
circDIAPH1 (hsa_circ_0074323) is highly expressed in CRC liver metastasis-associated samples and is proposed as a biomarker for CRLM. Functionally, circDIAPH1 promotes CRC cell proliferation, invasion, migration, tumor growth and liver metastasis while suppressing apoptosis. Mechanistically, circDIAPH1 recruits FOXA1 to activate CEACAM6 expression, and its own expression is regulated by the transcription factor BRD4. |
| Confidence score: |
0.8177 |