circRNA basic information
circBase ID: hsa_circ_0112136
Name: hsa_circ_NVL
Synonym: -
Host Gene: NVL
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
10534
Disease MeSH ID:
-
Disease NCIt ID:
C9331
Disease ICD11 ID:
1397617262
Disease OMIM ID:
613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissue; paired adjacent normal samples; tumor tissues

Cell lines:

AGS; HGC-27

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: FTO, PI3K, AKT, mTOR
Associated microRNA: -
Biological function: hsa_circ_0112136 functions as a tumor promoter in gastric cancer; its suppression reduced GC cell viability, migration, invasion and delayed tumor growth in vivo.
Molecular mechanism: FTO inhibits m6A modification of hsa_circ_0112136, thereby enhancing hsa_circ_0112136 expression; hsa_circ_0112136 promotes GC tumorigenesis by activating the PI3K/AKT/mTOR signaling pathway.
Biological pathway or process:

PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); m6A modification (other)

Detected method:
Q
M
Validation methods:

RT-qPCR; Microarray; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); MeRIP / MeRIP-seq; Western Blot; Bioinformatics Analysis

Clinical significance:

High hsa_circ_0112136 expression was correlated with larger tumor size, advanced TNM stage, poor differentiation, and distant metastasis; it may serve as a novel therapeutic target or biomarker for GC diagnosis and treatment.

Description:

hsa_circ_0112136 is up-regulated in gastric cancer and acts as an oncogenic circRNA. FTO-mediated demethylation decreases m6A modification of hsa_circ_0112136 and increases its expression, thereby promoting GC cell viability, migration, invasion and xenograft tumor growth through activation of the PI3K/AKT/mTOR pathway.

Confidence score:

0.795

Other information
Title:

Regulation of hsa_circ_0112136 by m6A demethylase FTO can enhance the malignancy of gastric cancer via the regulation of the PI3K/AKT/mTOR pathway.

Journal: Biotechnology and applied biochemistry
Published: 2024
PubMed ID: 39645568
Study type:

combined biological and clinical study

Data availability: GSE93541; Table S1; All data that had been produced and/or analyzed in the duration of this research have been appended in this manuscript.
Code availability: -