| Expression pattern: |
UP |
| Associated gene: |
PLEK2 |
| Associated microRNA: |
miR-138-5p |
| Biological function: |
Promotes NSCLC malignant phenotype, tumor growth and Warburg effect; promotes proliferation, migration, invasion, glucose uptake and lactate production; inhibits apoptosis. |
| Molecular mechanism: |
circCNKSR2 functions as a miR-138-5p sponge and indirectly modulates PLEK2 expression, thereby promoting NSCLC progression and Warburg effect. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circCNKSR2 may serve as a potential molecular biomarker and diagnostic marker for NSCLC. |
| Description: |
circCNKSR2 is up-regulated in NSCLC tissues and cells and mainly localized in the cytoplasm. It promotes NSCLC tumorigenesis, malignant cell behaviors and Warburg effect by sponging miR-138-5p and thereby increasing PLEK2 expression. The study suggests circCNKSR2 may be a diagnostic biomarker and therapeutic target for NSCLC. |
| Confidence score: |
0.7795 |