| Expression pattern: |
UP |
| Associated gene: |
PTEN, eIF4A3, FARSA pre-mRNA |
| Associated microRNA: |
- |
| Biological function: |
Promotes NSCLC cell migration, invasion, EMT and metastasis; induces macrophage polarization to the M2 phenotype. |
| Molecular mechanism: |
Exosomal circFARSA promotes PTEN ubiquitination and degradation, activates the PI3K/AKT signaling pathway, and induces M2 macrophage polarization; eIF4A3 promotes circFARSA biogenesis by binding to flanking sequences. |
| Biological pathway or process: |
PI3K/AKT (promotes); EMT (promotes); migration (promotes); invasion (promotes); metastasis (promotes); macrophage polarization (promotes); immune regulation (promotes); ubiquitination (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Exosomal circFARSA is proposed as a promising diagnostic and prognostic biomarker for NSCLC. |
| Description: |
circFARSA is upregulated in NSCLC tissues and cell lines and promotes NSCLC migration, invasion, EMT and metastasis. Tumor-derived exosomal circFARSA is taken up by macrophages, induces M2 polarization through PTEN ubiquitination/degradation and PI3K/AKT activation, and may serve as a diagnostic/prognostic biomarker for NSCLC. |
| Confidence score: |
0.8271 |