| Expression pattern: |
UP |
| Associated gene: |
CPA4, Ago2 |
| Associated microRNA: |
let‐7 |
| Biological function: |
promotes glioma cell proliferation and metastasis |
| Molecular mechanism: |
circCPA4 acts as a ceRNA by sponging let-7 to regulate CPA4 expression via RISC/Ago2, promoting glioma progression |
| Biological pathway or process: |
proliferation (promotes); metastasis (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; IHC (Immunohistochemistry); Survival Analysis; Clinical Sample Validation |
| Clinical significance: |
high levels of circCPA4 were related to poor outcome of glioma |
| Description: |
circCPA4 (hsa_circ_0082374), derived from CPA4, is up-regulated in glioma tissues and its high expression is associated with worse overall survival. Functionally, circCPA4 promotes glioma proliferation and metastasis. Mechanistically, it predominantly localizes in the cytoplasm and acts as a ceRNA sponge for let-7 to increase CPA4 expression via an Ago2/RISC-associated process. |
| Confidence score: |
0.7925 |