| Expression pattern: |
UP |
| Associated gene: |
G3BP2 |
| Associated microRNA: |
miR-217 |
| Biological function: |
promotes paclitaxel resistance, stemness, migration/metastasis-related behavior, and angiogenesis in breast cancer cells |
| Molecular mechanism: |
Exosomal circBACH1 acts as a miRNA sponge for miR-217, thereby upregulating G3BP2. |
| Biological pathway or process: |
chemoresistance (promotes); stemness (promotes); migration (promotes); angiogenesis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
B
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Western Blot; Transwell Assay; Tube Formation Assay |
| Clinical significance: |
circBACH1 is increased in BC tissues and in plasma exosomes of BC patients after PTX treatment, suggesting potential as a therapeutic target for PTX-resistance. |
| Description: |
circBACH1 is upregulated in paclitaxel-induced breast-cancer-derived exosomes and in breast cancer tissues, and it promotes PTX resistance, stemness, migration and angiogenesis. Mechanistically, exosomal circBACH1 acts as a ceRNA that sponges miR-217 to increase G3BP2 expression (circBACH1/miR-217/G3BP2). |
| Confidence score: |
0.7786 |