| Expression pattern: |
UP |
| Associated gene: |
PKM2 |
| Associated microRNA: |
miR-330-3p |
| Biological function: |
Promotes mesangial cell proliferation, enhances aerobic glycolysis/Warburg effect, increases glucose uptake, ATP production and lactate generation, and is associated with renal damage and IgAN pathogenesis. |
| Molecular mechanism: |
Acts as a sponge for miR-330-3p, thereby derepressing PKM2 expression and promoting PKM2-mediated aerobic glycolysis and mesangial cell proliferation. |
| Biological pathway or process: |
proliferation (promotes); glycolysis (promotes); ceRNA regulation (promotes); apoptosis (inhibits); cell cycle (promotes); fibrosis (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
Potential biomarker and therapeutic target; circRNA-0013747 expression positively correlates with blood/serum creatinine levels in IgAN patients. |
| Description: |
hsa_circ_0013747 is up-regulated in human IgAN kidney tissues and promotes mesangial cell proliferation and aerobic glycolysis. Mechanistically, it sponges miR-330-3p to derepress PKM2 expression, forming a circRNA_0013747/miR-330-3p/PKM2 axis with potential biomarker and therapeutic relevance. |
| Confidence score: |
0.8439 |