| Expression pattern: |
DN |
| Associated gene: |
ITCH, Dvl2, beta-catenin, Wnt3alpha, c-myc, cyclinD1 |
| Associated microRNA: |
miR-7, miR-214 |
| Biological function: |
circRNA-ITCH acts as a tumor suppressor in HCC cells; overexpression inhibits cell proliferation and colony formation and induces apoptosis. |
| Molecular mechanism: |
circRNA-ITCH interacts with miR-7 and miR-214 and inhibits Wnt/beta-catenin signal transduction, reducing beta-catenin protein and suppressing c-myc and cyclinD1 expression. |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); proliferation (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; Western Blot |
| Clinical significance: |
may become a biomarker for the diagnosis and risk prediction of HCC; may provide a theoretical basis for new therapeutic strategies for HCC |
| Description: |
circRNA-ITCH is down-regulated in human HCC cell lines compared with normal liver cells. Its overexpression suppresses HCC cell proliferation and colony formation and promotes apoptosis, at least partly by interacting with miR-7/miR-214 and inhibiting Wnt/beta-catenin signaling with reduced beta-catenin, c-myc, and cyclinD1 activity. The study proposes circRNA-ITCH as a potential biomarker and therapeutic target for HCC. |
| Confidence score: |
0.528 |