| Expression pattern: |
DN |
| Associated gene: |
RAF1 |
| Associated microRNA: |
miR-654-3p |
| Biological function: |
circRNA OMA1 overexpression alleviates DSS-induced colonic epithelial cell injury by enhancing cell viability, reducing apoptosis, decreasing cleaved caspase-3, and suppressing inflammatory cytokine secretion. |
| Molecular mechanism: |
circRNA OMA1 acts as a sponge for miR-654-3p and alleviates DSS-induced apoptosis and inflammation by modulating the miR-654-3p/RAF1 axis. |
| Biological pathway or process: |
apoptosis (inhibits); inflammation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; ELISA |
| Clinical significance: |
circRNA OMA1 could be a promising biomarker for the diagnosis and treatment of IBD. |
| Description: |
circRNA OMA1 is downregulated in the serum of children with IBD and in DSS-treated HT-29 cells. Its overexpression protects against DSS-induced colonic epithelial injury by sponging miR-654-3p, thereby modulating RAF1 expression, reducing apoptosis and inflammatory cytokine release, and improving cell viability. The study suggests circRNA OMA1 as a potential biomarker and therapeutic target for pediatric IBD. |
| Confidence score: |
0.6521 |