| Expression pattern: |
DN |
| Associated gene: |
ZNF131-354aa, TRIM28, CTBP2, E-cadherin, PTEN |
| Associated microRNA: |
- |
| Biological function: |
circZNF131 acts as a tumor suppressor in gastric cancer, inhibiting GC cell proliferation, migration and invasion; its encoded protein ZNF131-354aa inhibits GC progression in vitro and in vivo. |
| Molecular mechanism: |
circZNF131 encodes the IRES-driven protein ZNF131-354aa, which binds the intronic region of CTBP2 and suppresses CTBP2 transcription, thereby increasing E-cadherin and PTEN expression; ZNF131-354aa is regulated by TRIM28-mediated ubiquitination and degradation. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); cell cycle (other); PI3K/AKT (inhibits); ubiquitination (other); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Cell Cycle Assay; In Vivo Animal Model; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
circZNF131 is downregulated in plasma and tumor tissue of GC patients; low circZNF131 expression correlates with TNM stages, CEA, CA19-9, E-cadherin, Ki-67 and poor prognosis, suggesting potential clinical significance. |
| Description: |
circZNF131 is downregulated in gastric cancer plasma and tumor tissues, and low expression is associated with poor prognosis. Functionally, circZNF131 suppresses GC cell proliferation, migration, invasion and tumor growth by encoding the IRES-driven protein ZNF131-354aa. Mechanistically, ZNF131-354aa represses CTBP2 transcription to increase E-cadherin and PTEN expression, while TRIM28 promotes ZNF131-354aa ubiquitination and degradation. |
| Confidence score: |
0.89 |