| Expression pattern: |
UP |
| Associated gene: |
AGO2, SP1, PDIA4 promoter |
| Associated microRNA: |
miR-9-5p |
| Biological function: |
promotes proliferation, migration, invasion, and in vivo tumor growth in colorectal cancer |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-9-5p to upregulate SP1; SP1 binds PDIA4 promoter to induce circPDIA4 transcription, forming a positive feedback loop |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; ChIP / ChIP-seq; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
suggested as a promising biomarker for CRC patient treatment |
| Description: |
circPDIA4 is up-regulated in colorectal cancer tissues and cell lines and promotes CRC proliferation, migration, invasion, and xenograft tumor growth. Mechanistically, it mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-9-5p to de-repress SP1, while SP1 transcriptionally enhances PDIA4/circPDIA4, forming a positive feedback loop that aggravates CRC progression. |
| Confidence score: |
0.8455 |