| Expression pattern: |
UP |
| Associated gene: |
MCL-1 |
| Associated microRNA: |
miR-29c-3p |
| Biological function: |
promotes NSCLC cell growth/proliferation and clonogenicity |
| Molecular mechanism: |
circACC1 acts as a miRNA sponge by directly binding miR-29c-3p, thereby regulating the downstream target MCL-1 to promote NSCLC proliferation. |
| Biological pathway or process: |
proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Western Blot; Survival Analysis |
| Clinical significance: |
circACC1 is upregulated in NSCLC patients and negatively correlated with overall survival (OS). |
| Description: |
circACC1 is upregulated in NSCLC tissues and cell lines and promotes NSCLC cell proliferation and clonogenicity. Mechanistically, circACC1 sponges miR-29c-3p to increase MCL-1 expression, forming a circACC1/miR-29c-3p/MCL-1 regulatory axis associated with poor overall survival. |
| Confidence score: |
0.7507 |