circRNA basic information
circBase ID: hsa_circ_0003176
Name: -
Synonym: -
Host Gene: -
Genomic location(hg19): chr12:114823280-114841741:-
Genomic location(hg38): chr12:114385475-114403936:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: cisplatin-resistant non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

A549; H1299; A549-DDP; H1299-DDP
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: METTL3, RPS6KB1 mRNA, RPS6KB1
Associated microRNA: -
Biological function: Functions as a tumor suppressor in DDP-resistant NSCLC by suppressing proliferation, promoting apoptosis and autophagy, inhibiting glycolysis, suppressing tumor growth, and reversing or enhancing sensitivity to cisplatin.
Molecular mechanism: METTL3-mediated m6A modification suppresses hsa_circ_0003176 expression and reduces its stability; hsa_circ_0003176 directly binds to and destabilizes RPS6KB1 mRNA, attenuating mTORC1 signaling and regulating autophagy, glycolysis, apoptosis, proliferation, and DDP resistance.
Biological pathway or process:

autophagy (promotes); glycolysis (inhibits); apoptosis (promotes); proliferation (inhibits); m6A modification (other); mRNA stability (inhibits); drug resistance (inhibits); chemoresistance (inhibits); other pathway/process (inhibits)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Bioinformatics Analysis; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); Actinomycin D / DRB Stability Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model
Clinical significance:

-
Description:

hsa_circ_0003176 is downregulated in DDP-resistant NSCLC cells and acts as a tumor suppressor that inhibits proliferation and glycolysis, promotes apoptosis and autophagy, and reverses cisplatin resistance. METTL3-mediated m6A modification suppresses hsa_circ_0003176 expression, while hsa_circ_0003176 directly destabilizes RPS6KB1 mRNA to attenuate mTORC1-related malignant and chemoresistant phenotypes.
Confidence score:

0.7609
Other information
Title:

Hsa_circ_0003176: a key player in the m6A modification-mediated regulation of autophagy and glycolysis in cisplatin-resistant non-small cell lung cancer.

Journal: Human cell
Published: 2026
PubMed ID: 41579303
Study type:

biological research
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request; supplementary material available at https:// doi. org/ 10. 1007/ s13577-026-01350-y
Code availability: -