circRNA basic information
circBase ID: hsa_circ_0075402
Name: hsa_circ_RACK1
Synonym: circRACK1
Host Gene: RACK1
Genomic location(hg19): chr5:180668491-180669345:-
Genomic location(hg38): chr5:181241491-181242345:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; lung; liver
Cell lines:

GES-1; HGC27; MKN-28; AGS; MKN-45; MGC-803; BGC-823; HUVEC; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: Vimentin, ACTIN, HIF-1alpha, circR-127aa
Associated microRNA: -
Biological function: circRACK1 acts as a tumor suppressor in gastric cancer: its overexpression inhibits proliferation, colony formation, migration, invasion, angiogenesis, actin-cytoskeletal remodeling, EMT, tumor growth and metastasis, while promoting apoptosis; its knockdown has the opposite effects.
Molecular mechanism: circRACK1 encodes the peptide circR-127aa, which interacts with Vimentin and ACTIN, promotes ubiquitin-mediated proteasomal degradation of Vimentin, and thereby suppresses actin-cytoskeletal remodeling and EMT; HIF-1alpha can mitigate circRACK1 effects under hypoxic conditions.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); angiogenesis (inhibits); apoptosis (promotes); EMT (inhibits); metastasis (inhibits); ubiquitination (promotes); HIF-1 signaling (other); VEGF/VEGFR (inhibits); other pathway/process (inhibits)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Western Blot; IF (Immunofluorescence); Co-IP; RNA Pull-Down; Transfection; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Tube Formation Assay; IHC (Immunohistochemistry); H&E Staining; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

circRACK1 and its encoded peptide circR-127aa may serve as potential biomarkers and therapeutic targets for gastric cancer.
Description:

hsa_circ_0075402/circRACK1 is down-regulated in gastric cancer cells and encodes the tumor-suppressive peptide circR-127aa. circR-127aa interacts with Vimentin and ACTIN, promotes Vimentin ubiquitination and proteasomal degradation, and inhibits proliferation, migration, invasion, angiogenesis, EMT, tumor growth and metastasis while promoting apoptosis.
Confidence score:

0.8189
Other information
Title:

Inhibitory effects of circR-127aa on gastric cancer progression and tumor growth.

Journal: Cellular signalling
Published: 2025
PubMed ID: 39581359
Study type:

biological research
Data availability: Data will be made available on request; Supplementary data: https://doi.org/10.1016/j.cellsig.2024.111520
Code availability: -