circRNA basic information
circBase ID: hsa_circ_0001681
Name: hsa_circ_RAPGEF5
Synonym: -
Host Gene: RAPGEF5
Genomic location(hg19): chr7:22330793-22357656:-
Genomic location(hg38): chr7:22291174-22318037:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0021042
MONDO name: glioma
Disease details: gliomas
Disease DO ID:
-
Disease MeSH ID:
D005910
Disease NCIt ID:
C3059
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissue samples; control non-neoplastic brain tissue samples

Cell lines:

HEB; U87; U251; A172; T98G

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: 2791 potential target mRNAs
Associated microRNA: hsa-miR-136
Biological function: inhibits glioma carcinogenesis; suppresses glioma cell proliferation, migration and invasion; suppresses intracranial tumor growth and prolongs survival in vivo
Molecular mechanism: ceRNA mechanism through interaction with miRNAs and downstream target mRNAs; predicted modulation of PI3K-Akt, MAPK and Ras signaling pathways
Biological pathway or process:

ceRNA regulation (other); PI3K/AKT (inhibits); MAPK (inhibits); apoptosis (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); other pathway/process (inhibits)

Detected method:
Q
S
Validation methods:

RNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model

Clinical significance:

potential diagnostic and therapeutic biomarker; candidate molecule for therapeutic strategies

Description:

hsa_circ_0001681 is a down-regulated circRNA in glioma and was experimentally validated as a circular transcript derived from RAPGEF5. Its overexpression suppresses glioma cell proliferation, migration, invasion and intracranial tumor growth, likely through a circRNA-miRNA-mRNA ceRNA network affecting PI3K-Akt, MAPK and Ras-related signaling.

Confidence score:

0.6867

Other information
Title:

Identification of a functional CircRNA-miRNA-mRNA network and inhibitory effect of Hsa_circ_0001681 on gliomas.

Journal: Biochemical and biophysical research communications
Published: 2025
PubMed ID: 39818187
Study type:

combined biological and bioinformatics study

Data availability: GSE165926; Supplementary Table 1A; Supplementary Table 3; https://doi.org/10.1016/j.bbrc.2024.151236
Code availability: -