circRNA basic information
circBase ID: -
Name: hsa_circ_FOXO3
Synonym: circFOXO3
Host Gene: FOXO3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005076
MONDO name: periodontitis
Disease details: periodontitis
Disease DO ID:
824, 9893
Disease MeSH ID:
D010518
Disease NCIt ID:
C34918
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

gingival epithelial tissues; gingival epithelium; gingival tissues

Cell lines:

HaCaT; IHGK; 293T

In vivo animal model:

other disease animal model

circRNA-disease information
Expression pattern:
UP
Associated gene: FOXO3, JunB, ITGbeta6, TGF-beta, p-Smad2, Ago2
Associated microRNA: miR-141-3p
Biological function: Promotes periodontal inflammation and periodontitis progression; down-regulates ITGbeta6 expression; suppresses TGF-beta activity; circFOXO3 inhibition alleviates alveolar bone resorption and periodontal inflammation in rats.
Molecular mechanism: circFOXO3 acts as an miR-141-3p sponge, increases FOXO3, promotes FOXO3-JunB transcriptional repression, inhibits JunB binding to the ITGbeta6 promoter, down-regulates ITGbeta6 transcription, and reduces ITGbeta6-mediated TGF-beta/Smad2 activity.
Biological pathway or process:

inflammation (promotes); TGF-beta/SMAD (inhibits); ceRNA regulation (other); other pathway/process (other)

Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Co-IP; ChIP / ChIP-seq; IF (Immunofluorescence); IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis

Clinical significance:

Potential therapeutic target for periodontitis; local administration of anti-circFOXO3 small RNAs showed preventive and therapeutic efficacy against periodontal inflammation and alveolar bone resorption in rats.

Description:

circFOXO3 is up-regulated in gingival epithelial tissues from patients with periodontitis and is mainly cytoplasmic. It promotes periodontal inflammation by sponging miR-141-3p, increasing FOXO3, forming a FOXO3-JunB transcriptional repression mechanism, down-regulating ITGbeta6, and inhibiting TGF-beta/Smad2 activity. In a rat ligature-induced periodontitis model, local si-circFOXO3 treatment alleviated inflammation and alveolar bone resorption, suggesting therapeutic potential.

Confidence score:

0.8729

Other information
Title:

Targeting circFOXO3 to Modulate Integrin beta6 Expression in Periodontitis: A Potential Therapeutic Approach.

Journal: Journal of clinical periodontology
Published: 2025
PubMed ID: 39800368
Study type:

combined biological and clinical study

Data availability: The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.
Code availability: -