| Expression pattern: |
UP |
| Associated gene: |
190 mRNAs, SLC7A11, GPX4, Bax, caspase-3, vimentin, N-cadherin, E-cadherin |
| Associated microRNA: |
hsa-miR-940, hsa-miR-326, hsa-miR-657, hsa-miR-330-5p, hsa-miR-615-5p, hsa-miR-375 |
| Biological function: |
Promotes osteosarcoma cell proliferation, migration, invasion, lung metastasis, and EMT progression. |
| Molecular mechanism: |
hsa_circ_0002005 may function through a predicted circRNA-miRNA-mRNA interaction network and by modulating EMT-associated proteins, including increased N-cadherin and vimentin and decreased E-cadherin. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (other); apoptosis (other); ferroptosis (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0002005 could potentially act as a biomarker for diagnosing osteosarcoma and a therapeutic target. |
| Description: |
hsa_circ_0002005 is a MIER2-derived human circRNA that is up-regulated in osteosarcoma tissues and cell lines. Functional knockdown experiments show that it promotes OS cell proliferation, migration, invasion, EMT, and lung metastasis in vivo. The study suggests that hsa_circ_0002005 may act through a predicted circRNA-miRNA-mRNA network and may serve as a diagnostic biomarker or therapeutic target for OS. |
| Confidence score: |
0.7761 |