circRNA basic information
circBase ID: -
Name: hsa_circ_FADS2
Synonym: hsa_circRNA_100833 / circFADS2
Host Gene: FADS2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005229
MONDO name: bacterial infectious disease with sepsis
Disease details: sepsis
Disease DO ID:
 0040085
Disease MeSH ID:
 D016470
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

plasma
Cell lines:

HBEpCs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: -
Associated microRNA: miR-133a
Biological function: Protects LPS-induced HBEpCs from apoptosis and promotes cell viability; reduces inflammatory cytokine production in LPS-treated HBEpCs.
Molecular mechanism: Acts as a sponge/target of miR-133a; circFADS2 overexpression decreases miR-133a expression and reverses miR-133a-mediated effects on apoptosis/viability.
Biological pathway or process:

apoptosis (inhibits); proliferation (promotes); inflammation (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; ELISA
Clinical significance:

CircFADS2 is downregulated in plasma from sepsis patients compared with healthy controls.
Description:

circFADS2 is downregulated in sepsis plasma and in LPS-treated human bronchial epithelial cells. Functionally, circFADS2 overexpression protects HBEpCs from LPS-induced apoptosis and loss of viability by sponging/downregulating miR-133a, supported by luciferase and gain/loss-of-function assays.
Confidence score:

0.6521
Other information
Title:

CircRNA circFADS2 is under-expressed in sepsis and protects lung cells from LPS-induced apoptosis by downregulating miR-133a.

Journal: Journal of inflammation (London, England)
Published: 2022
PubMed ID: 35279129
Study type:

combined biological and clinical study
Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -