circRNA basic information
circBase ID: hsa_circ_0007933
Name: hsa_circ_MID1
Synonym: -
Host Gene: MID1
Genomic location(hg19): chrX:10534927-10535643:-
Genomic location(hg38): chrX:10566887-10567603:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer / PCa
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PCa tissues; prostatectomy tissues of BPH patients
Cell lines:

PC-3; DU145; RWPE-1; 293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: IGF1R, YTHDC2, Ago2, AKT
Associated microRNA: miR-330-3p
Biological function: promotes PCa cell proliferation, migration, invasion and glycolysis; promotes tumorigenesis in vivo
Molecular mechanism: circMID1 acts as a miRNA sponge for miR-330-3p, relieving repression of YTHDC2 and IGF1R and activating the YTHDC2/IGF1R/AKT axis
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); PI3K/AKT (promotes); cell cycle (promotes)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; RT-qPCR; Clinical Sample Validation; Transfection; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis; Microarray
Clinical significance:

High circMID1 expression was related to Gleason score, pathological stage, and whether bone metastasis occurs in PCa patients.
Description:

circMID1 (hsa_circ_0007933), derived from the MID1 gene, is up-regulated in prostate cancer tissues and cell lines and mainly localizes to the cytoplasm. It promotes proliferation, cell-cycle progression, migration, invasion and glycolysis and enhances xenograft tumor growth. Mechanistically, circMID1 sponges miR-330-3p to increase YTHDC2 and IGF1R expression, activating the IGF1R/AKT signaling axis.
Confidence score:

0.8635
Other information
Title:

Circular RNA midline-1 (circMID1) promotes proliferation, migration, invasion and glycolysis in prostate cancer.

Journal: Bioengineered
Published: 2022
PubMed ID: 35212614
Study type:

combined biological and clinical study
Data availability: GSE113153
Code availability: -