| Expression pattern: |
UP |
| Associated gene: |
GLUT1, glucose transporter 1, glucose transporter protein, SLC2A1, BAX, BCL2, Ki67 |
| Associated microRNA: |
let-7b-5p |
| Biological function: |
Promotes MM cell proliferation, colony formation, glycolysis, BTZ resistance and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circ_0003489 acts as a ceRNA by sponging let-7b-5p, thereby mediating GLUT1 expression and promoting MM progression and BTZ resistance. |
| Biological pathway or process: |
proliferation (promotes); glycolysis (promotes); apoptosis (inhibits); drug resistance (promotes); chemoresistance (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ_0003489 was overexpressed in MM patient bone marrow samples, associated with the percentage of myeloma cells in bone marrow, and proposed as a promising therapeutic target for MM. |
| Description: |
hsa_circ_0003489/circ_0003489 is up-regulated in bone marrow samples from MM patients and mainly localizes to the cytoplasm of MM cells. It promotes MM cell proliferation, glycolysis, BTZ resistance and in vivo tumor growth while inhibiting apoptosis, mechanistically acting through the circ_0003489/let-7b-5p/GLUT1 axis. The study proposes circ_0003489 as a potential therapeutic target for MM progression and chemoresistance. |
| Confidence score: |
0.8254 |