| Expression pattern: |
UP |
| Associated gene: |
DAPK1, DAPK1 3 ′ UTR, DAPK1 mRNA |
| Associated microRNA: |
miR-26a-5p |
| Biological function: |
circTLK1 promotes dopaminergic neuron injury, apoptosis and cytotoxicity in PD models, while circTLK1 knockdown improves cell viability, suppresses apoptosis and cytotoxicity, and ameliorates neuron damage and motor dysfunction. |
| Molecular mechanism: |
circTLK1 regulates dopaminergic neuron injury through a ceRNA-like circTLK1/miR-26a-5p/DAPK1 axis, interacting with miR-26a-5p and thereby modulating DAPK1 expression. |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; CCK8; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; TUNEL; IHC (Immunohistochemistry); H&E Staining; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Targeting circTLK1 may contribute to improving PD therapy. |
| Description: |
circTLK1 is up-regulated in PD-related in vivo and in vitro dopaminergic neuron injury models. Its knockdown alleviates neuronal injury by improving viability and reducing apoptosis and cytotoxicity, mechanistically through the circTLK1/miR-26a-5p/DAPK1 regulatory axis. The study suggests circTLK1 as a potential therapeutic target for PD. |
| Confidence score: |
0.6542 |