| Expression pattern: |
UP |
| Associated gene: |
TGF-beta-activated kinase 1/MAP3K7 binding protein 3 (TAB3) mRNA, TAB3, NF-kappaB p65, p-p65 |
| Associated microRNA: |
miR-23b-3p |
| Biological function: |
circXPO1 promotes cigarette smoke/CSE-induced inflammatory cytokine production and cellular senescence in alveolar epithelial cells; circXPO1 knockdown attenuates inflammation and senescence in vitro and in vivo. |
| Molecular mechanism: |
circXPO1 acts as a competing endogenous RNA that sponges miR-23b-3p, thereby releasing/upregulating TAB3 and activating NF-kappaB signaling under cigarette smoke/CSE conditions. |
| Biological pathway or process: |
NF-kappaB (promotes); inflammation (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; Luciferase Reporter Assay; ELISA; Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
circXPO1 intervention is proposed as a potential therapeutic target for reducing inflammation and cellular senescence in COPD, but no patient clinical validation was performed. |
| Description: |
circXPO1 is up-regulated in CS-exposed mouse lungs and CSE-treated MLE12 alveolar epithelial cells. It promotes inflammatory cytokine production and cellular senescence by sponging miR-23b-3p, releasing TAB3 and activating NF-kappaB signaling; knockdown of circXPO1 attenuates these COPD-related phenotypes in vitro and in vivo. |
| Confidence score: |
0.6867 |