| Expression pattern: |
UP |
| Associated gene: |
GATA6 |
| Associated microRNA: |
miR-326 |
| Biological function: |
Promotes breast cancer cell proliferation, angiogenesis, migration, invasion, EMT, tumor growth and metastasis; inhibits apoptosis. |
| Molecular mechanism: |
circ_0008717 acts as a cytoplasmic ceRNA that sponges miR-326 to upregulate GATA6 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); angiogenesis (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0008717 expression was associated with shorter overall survival and may serve as a prognostic and therapeutic target in breast cancer. |
| Description: |
circ_0008717 is up-regulated in breast cancer tissues and cells and high expression is associated with shorter overall survival. It functions as an oncogenic cytoplasmic ceRNA by sponging miR-326, thereby elevating GATA6 expression to promote proliferation, angiogenesis, migration, invasion, EMT, tumor growth and metastasis while suppressing apoptosis. |
| Confidence score: |
0.8354 |