| Expression pattern: |
UP |
| Associated gene: |
PABPC1, STMN1 |
| Associated microRNA: |
- |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion, EMT, tumor growth and lung metastasis. |
| Molecular mechanism: |
circFOXK2 binds PABPC1 to form a circRNA-protein complex that stabilizes STMN1 mRNA (not via miRNA sponge/ceRNA). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); mRNA stability (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; divergent primers PCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; ROC Analysis; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circFOXK2 had diagnostic value in NSCLC tissues (AUC = 0.7091). |
| Description: |
circFOXK2 (hsa_circ_0046430) is up-regulated in NSCLC tissues and cell lines and promotes proliferation, migration, invasion, EMT and in vivo tumor growth/metastasis. Mechanistically, it localizes mainly to the cytoplasm and forms a complex with the RBP PABPC1 to stabilize STMN1 mRNA, thereby increasing STMN1 expression; the effect is not mediated by miRNA sponging. |
| Confidence score: |
0.8932 |