| Expression pattern: |
DN |
| Associated gene: |
EIF4A3, SHPRH pre-mRNA, IL-17A, IL-17F |
| Associated microRNA: |
- |
| Biological function: |
Functions as a tumor suppressor in RB; overexpression inhibits RB cell proliferation, migration, invasion and tumor growth, and promotes apoptosis. |
| Molecular mechanism: |
EIF4A3 suppresses hsa_circ_0078136 expression by binding to sequences in the flanking region of SHPRH pre-mRNA; hsa_circ_0078136 suppresses RB tumorigenesis via inhibition of the IL-17 signaling pathway. |
| Biological pathway or process: |
other pathway/process (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic tool for mitigating RB progression. |
| Description: |
hsa_circ_0078136 is down-regulated in retinoblastoma tissues and cell lines and acts as a tumor suppressor. Its overexpression inhibits RB cell proliferation, migration, invasion, and xenograft tumor growth while promoting apoptosis, partly through suppression of IL-17 signaling. EIF4A3 negatively regulates hsa_circ_0078136 formation by binding flanking sequences in SHPRH pre-mRNA. |
| Confidence score: |
0.7404 |