| Expression pattern: |
DN |
| Associated gene: |
MKLN1 mRNA, MKLN1, HuR |
| Associated microRNA: |
- |
| Biological function: |
Circ_0082415 acts as a tumor suppressor in retinoblastoma cells by inhibiting RB cell viability, proliferation, migration and invasion, and promoting apoptosis. |
| Molecular mechanism: |
Circ_0082415 binds HuR and reduces HuR binding to MKLN1 mRNA, thereby suppressing HuR-mediated MKLN1 mRNA translation and decreasing MKLN1 protein output. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
Circ_0082415 may provide a potential therapeutic target and a novel view for the treatment of retinoblastoma. |
| Description: |
MKLN1-derived hsa_circ_0082415 is down-regulated in retinoblastoma cells. Its overexpression suppresses RB cell growth, proliferation, migration and invasion while promoting apoptosis. Mechanistically, circ_0082415 interacts with HuR and inhibits HuR-mediated MKLN1 mRNA translation, reducing MKLN1 protein output and suppressing RB progression. |
| Confidence score: |
0.7812 |