circRNA basic information
circBase ID: hsa_circ_0077892
Name: hsa_circ_TBPL1
Synonym: circTBPL1
Host Gene: TBPL1
Genomic location(hg19): chr6:134301219-134305809:+
Genomic location(hg38): chr6:133980081-133984671:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; adjacent normal tissues; tumor tissues
Cell lines:

MDA-MB-231; MDA-MB-468; HEK293T; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: TPBG, AGO2
Associated microRNA: miR-653-5p
Biological function: promotes proliferation, migration, invasion, EMT, angiogenesis, tumor growth and metastasis in breast cancer
Molecular mechanism: Exosomal circTBPL1 from CAFs is transferred to breast cancer cells and functions as a ceRNA by sponging miR-653-5p, thereby relieving miR-653-5p-mediated repression of TPBG.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); angiogenesis (promotes); ceRNA regulation (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; ISH (In Situ Hybridization); RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

potential biomarker and novel therapeutic target for breast cancer
Description:

circTBPL1 (hsa_circ_0077892) is enriched and upregulated in CAF-derived exosomes and can be transferred to breast cancer cells. It promotes malignant phenotypes (proliferation, migration/invasion, EMT, angiogenesis, tumor growth and metastasis) mainly by sponging miR-653-5p to derepress TPBG.
Confidence score:

0.872
Other information
Title:

Cancer-associated fibroblasts facilitate breast cancer progression through exosomal circTBPL1-mediated intercellular communication.

Journal: Cell death & disease
Published: 2023
PubMed ID: 37495592
Study type:

combined biological and clinical study
Data availability: All data are available from the corresponding author upon reasonable request.
Code availability: -