| Expression pattern: |
UP |
| Associated gene: |
Argonaute 2 (Ago2), FSCN1, S6K1 |
| Associated microRNA: |
miR-145 |
| Biological function: |
Promotes NSCLC cell growth, proliferation, migration and invasion; circBIRC6 silencing inhibits NSCLC xenograft growth in SCID mice. |
| Molecular mechanism: |
circBIRC6 acts through a ceRNA/miRNA sponging mechanism involving miR-145, leading to regulation of miR-145 targets FSCN1 and S6K1. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Transfection; EdU Staining; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; TUNEL; Western Blot; In Vivo Animal Model |
| Clinical significance: |
circBIRC6 may serve as a novel prognostic biomarker and therapeutic target for human NSCLC. |
| Description: |
This study found that human circBIRC6 is upregulated in NSCLC tissues and cells. Functionally, circBIRC6 promotes NSCLC cell growth, proliferation, migration and invasion, while circBIRC6 knockdown suppresses xenograft growth. Mechanistically, circBIRC6 likely sponges miR-145 and thereby modulates miR-145 targets including FSCN1 and S6K1. |
| Confidence score: |
0.6287 |