| Expression pattern: |
UP |
| Associated gene: |
GPX4 |
| Associated microRNA: |
miR-1287-5p |
| Biological function: |
Acts as an oncogene in NSCLC; promotes proliferation/viability, migration and invasion; inhibits apoptosis and ferroptosis; increases sensitivity to chemotherapeutic agents when silenced; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circDTL sponges miR-1287-5p to derepress GPX4, thereby inhibiting apoptosis and ferroptosis and promoting oncogenic phenotypes |
| Biological pathway or process: |
apoptosis (inhibits); ferroptosis (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); chemoresistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Wound Healing Assay; Transwell Assay; ELISA; Western Blot; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circDTL is up-regulated in NSCLC tissues/cell lines and functions as an oncogene. It mainly localizes to the cytoplasm and acts as a miRNA sponge for miR-1287-5p, thereby increasing GPX4 expression to suppress apoptosis and ferroptosis and promote malignant phenotypes; circDTL silencing enhances chemosensitivity and inhibits xenograft growth. |
| Confidence score: |
0.7099 |