circRNA basic information
circBase ID: hsa_circ_0000069
Name: hsa_circ_STIL
Synonym: circ0000069
Host Gene: STIL
Genomic location(hg19): chr1:47745912-47748131:-
Genomic location(hg38): chr1:47280240-47282459:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002974
MONDO name: cervical cancer
Disease details: cervical cancer
Disease DO ID:
 4362
Disease MeSH ID:
 -
Disease NCIt ID:
 C9311
Disease ICD11 ID:
 1256072522
Disease OMIM ID:
 603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

CC tissues; adjacent normal tissues; matched normal tissues; tumor tissues
Cell lines:

SiHa; Caski; C33A; Ect1; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: METTL3, STIL
Associated microRNA: miR-4426
Biological function: Promotes cervical cancer cell proliferation and migration and promotes cervical cancer tumor growth in vivo.
Molecular mechanism: m6A modification maintains circ0000069 stability; circ0000069 functions as a miR-4426 sponge to mediate pro-oncogenic effects in cervical cancer.
Biological pathway or process:

proliferation (promotes); migration (promotes); ceRNA regulation (other); m6A modification (other)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; MeRIP / MeRIP-seq; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Luciferase Reporter Assay; Nuclear-Cytoplasmic Fractionation; ROC Analysis; Bioinformatics Analysis
Clinical significance:

ROC analysis showed high accuracy for circ0000069 in CC diagnosis.
Description:

hsa_circ_0000069/circ0000069 is upregulated in cervical cancer cells and tissues and shows diagnostic potential by ROC analysis. m6A modification, involving METTL3-dependent regulation, maintains circ0000069 stability, and circ0000069 promotes proliferation, migration, and xenograft tumor growth by sponging miR-4426.
Confidence score:

0.8712
Other information
Title:

circ0000069 promotes cervical cancer cell proliferation and migration by inhibiting miR-4426.

Journal: Biochemical and biophysical research communications
Published: 2021
PubMed ID: 33725572
Study type:

combined biological and clinical study
Data availability: GSE113696; GSE102686; Supplementary data: https://doi.org/10.1016/j.bbrc.2021.03.020
Code availability: -