| Expression pattern: |
UP |
| Associated gene: |
METTL14, HuR, ASPH mRNA |
| Associated microRNA: |
- |
| Biological function: |
circARHGAP12 promotes iron overload-induced lipid peroxidative damage, decreases cell viability, increases apoptosis, and facilitates atherosclerosis progression. |
| Molecular mechanism: |
METTL14-mediated m6A modification enhances circARHGAP12 stability and expression; circARHGAP12 binds HuR to stabilize ASPH mRNA and promote ASPH transcription. |
| Biological pathway or process: |
m6A modification (other); mRNA stability (promotes); apoptosis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); Transfection; CCK8; TUNEL; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circARHGAP12 is up-regulated in atherosclerotic tissues and ox-LDL-treated HUVECs. METTL14-mediated m6A modification increases circARHGAP12 stability, and circARHGAP12 binds HuR to stabilize ASPH mRNA, promoting ASPH transcription and aggravating iron overload-related lipid peroxidative damage, apoptosis, and AS progression. |
| Confidence score: |
0.7394 |