| Expression pattern: |
UP |
| Associated gene: |
KIF2A, p-PI3K, p-Akt, Notch1 |
| Associated microRNA: |
miR-140-3p |
| Biological function: |
Promotes HCC cell proliferation and invasion, promotes tumor growth, inhibits oxaliplatin-induced apoptosis, and promotes chemotherapy resistance to oxaliplatin, doxorubicin, and sorafenib. |
| Molecular mechanism: |
hsa_circ_0088036 directly binds and negatively regulates miR-140-3p, thereby positively regulating KIF2A expression and activating PI3K/Akt and Notch signaling pathways. |
| Biological pathway or process: |
PI3K/AKT (promotes); Notch (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits); drug resistance (promotes); chemoresistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Higher expression of hsa_circ_0088036 was associated with tumor size and TNM stage in HCC patients, and hsa_circ_0088036 may be a potential therapeutic target for chemotherapy-resistant HCC. |
| Description: |
hsa_circ_0088036 is up-regulated in HCC tissues and cells and is even higher in oxaliplatin-resistant cells. It promotes HCC proliferation, invasion, tumor growth, and chemotherapy resistance by binding miR-140-3p, increasing KIF2A expression, and activating PI3K/Akt and Notch signaling, suggesting potential as a therapeutic target for chemotherapy-resistant HCC. |
| Confidence score: |
0.8005 |