| Expression pattern: |
UP |
| Associated gene: |
TRAF6, collagen II, MMP-13, ADAMTS-5, TNF-a, IL-6, cleaved caspase3, cleaved caspase-9, bcl-2 |
| Associated microRNA: |
miR-345-3p |
| Biological function: |
circRSU1 promotes IL-1beta-induced chondrocyte dysfunction in osteoarthritis, including inhibition of cell growth, extracellular matrix degradation, apoptosis, and pro-inflammatory cytokine production; circRSU1 knockdown reverses these impairments. |
| Molecular mechanism: |
circRSU1 acts through a ceRNA-like circRSU1/miR-345-3p/TRAF6 signaling mechanism; circRSU1 targets miR-345-3p, which regulates TRAF6, affecting chondrocyte proliferation, apoptosis, ECM degradation and inflammatory cytokine production. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); apoptosis (promotes); inflammation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; ELISA |
| Clinical significance: |
circRSU1 may provide novel insight for targeted therapies of osteoarthritis. |
| Description: |
circRSU1 is up-regulated in osteoarthritis specimens and in IL-1beta-treated human chondrocytes. Functional experiments showed that circRSU1 knockdown attenuates IL-1beta-induced chondrocyte dysfunction by restoring proliferation and reducing apoptosis, ECM degradation and inflammatory cytokine production. Mechanistically, circRSU1 regulates the miR-345-3p/TRAF6 signaling axis in OA progression. |
| Confidence score: |
0.6521 |