circRNA basic information
circBase ID: hsa_circ_0020123
Name: hsa_circ_PDZD8
Synonym: Circ_0020123
Host Gene: PDZD8
Genomic location(hg19): chr10:119042605-119049859:-
Genomic location(hg38): chr10:117283094-117290348:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung carcinomas
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; matched non-cancerous tissues; NSCLC tissues; paracarcinoma tissues
Cell lines:

BEAS-2B; A549; PC9
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: KIAA1522, Ago2
Associated microRNA: miR-940
Biological function: Circ_0020123 acts as an oncogene in NSCLC, promoting tumorigenesis, tumor growth, cell viability, colony formation, cell cycle progression, migration and invasion, while inhibiting apoptosis.
Molecular mechanism: Circ_0020123 functions as a ceRNA that directly binds miR-940 and up-regulates KIAA1522 expression through the miR-940/KIAA1522 axis.
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

Higher circ_0020123 expression was associated with advanced TNM stages, metastasis, tumor size, pathological type, clinical stage, differentiation, and lymph node metastasis; circ_0020123 siRNA was suggested as a potential therapeutic molecular for NSCLC molecular targeted therapy.
Description:

Circ_0020123 is up-regulated in NSCLC tissues and cell lines and is associated with more advanced clinicopathological features. It promotes NSCLC cell proliferation, cell cycle progression, migration, invasion and xenograft tumor growth while suppressing apoptosis. Mechanistically, cytoplasmic circ_0020123 acts as a ceRNA for miR-940, thereby increasing KIAA1522 expression through the circ_0020123/miR-940/KIAA1522 axis.
Confidence score:

0.8279
Other information
Title:

Circ_0020123 promotes NSCLC tumorigenesis via up-regulating KIAA1522 expression through miR-940.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2022
PubMed ID: 35196193
Study type:

combined biological and clinical study
Data availability: -
Code availability: -