| Expression pattern: |
UP |
| Associated gene: |
Akt / AKT2 |
| Associated microRNA: |
miR-326 |
| Biological function: |
promotes cervical cancer progression; inhibits apoptosis; promotes migration/metastasis; supports cell cycle progression and xenograft growth; promotes angiogenesis |
| Molecular mechanism: |
ceRNA/miRNA sponge: hsa_circRNA_0001400 sponges miR-326, thereby relieving miR-326-mediated repression of Akt/AKT2 and affecting p-Akt signaling |
| Biological pathway or process: |
apoptosis (inhibits); cell cycle (promotes); migration (promotes); metastasis (promotes); angiogenesis (promotes); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Colony Formation Assay; Transwell Assay; In Vivo Animal Model |
| Clinical significance: |
hsa_circRNA_0001400 might be used as novel diagnostic and prognostic biomarkers as well as for targeted therapies |
| Description: |
hsa_circRNA_0001400 is up-regulated in cervical cancer and functions as a miR-326 sponge to sustain Akt/AKT2 expression and p-Akt signaling, thereby promoting tumor progression. Silencing hsa_circRNA_0001400 increases miR-326, suppresses Akt, enhances apoptosis, inhibits migration, and reduces xenograft growth/angiogenesis. |
| Confidence score: |
0.6004 |