| Expression pattern: |
UP |
| Associated gene: |
CXCL5, VEGF-C, neutrophils |
| Associated microRNA: |
miR-149-5p |
| Biological function: |
Promotes lymphangiogenesis and lymph node metastasis; promotes migration, invasion and proliferation of bladder cancer cells. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-149-5p to relieve repression on CXCL5; circDHTKD1-induced CXCL5 recruits/activates neutrophils which secrete VEGF-C to promote lymphangiogenesis. |
| Biological pathway or process: |
ceRNA regulation (promotes); lymphangiogenesis (promotes); migration (promotes); invasion (promotes); proliferation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Sanger Sequencing; divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Clinical Sample Validation; Transfection; Wound Healing Assay; Transwell Assay; CCK8; Colony Formation Assay; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; ELISA; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circDHTKD1 expression was positively correlated with LN metastasis of BCa; proposed as a promising diagnostic and therapeutic target for LN metastasis in BCa. |
| Description: |
circDHTKD1 is up-regulated in bladder cancer and associated with lymph node metastasis. It promotes lymphangiogenesis and metastasis by sponging miR-149-5p to upregulate CXCL5, which recruits/activates neutrophils and increases VEGF-C to further enhance lymphangiogenesis. |
| Confidence score: |
0.8837 |