circRNA basic information
circBase ID: hsa_circ_0038632
Name: hsa_circ_PLK1
Synonym: circ_0038632
Host Gene: PLK1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
1612
Disease MeSH ID:
-
Disease NCIt ID:
C9335
Disease ICD11 ID:
1047754165
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary breast tumor specimens; adjacent noncancerous breast tissue; xenograft tumor tissues

Cell lines:

MCF-10A; MCF-7; MDA-MB-468

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: CDCA3, Ago2, Bax, Bcl-2, Ki67
Associated microRNA: miR-520a-3p
Biological function: Promotes breast cancer cell growth, viability, proliferation, motility, invasiveness, metastasis-related phenotypes and xenograft tumorigenicity, while inhibiting apoptosis.
Molecular mechanism: circ_0038632 functions as a ceRNA/miRNA sponge for miR-520a-3p and post-transcriptionally modulates CDCA3 expression through the miR-520a-3p/CDCA3 cascade.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Clinical Sample Validation; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)

Clinical significance:

circ_0038632 may be a potential target for BC treatment; previous studies reported that circ_0038632 is related to poor survival of BC patients.

Description:

circ_0038632 is up-regulated in breast cancer tissues and cell lines and acts as an oncogenic circRNA. It promotes BC cell proliferation, migration, invasion, and xenograft tumor growth while suppressing apoptosis by sponging miR-520a-3p and increasing CDCA3 expression. The study suggests circ_0038632 as a potential therapeutic target for breast cancer.

Confidence score:

0.8006

Other information
Title:

Identification of circ_0038632 as a Promoter of Breast Cancer through miR-520a-3p-Dependent Modulation of CDCA3.

Journal: Annals of clinical and laboratory science
Published: 2023
PubMed ID: 37625824
Study type:

combined biological and clinical study

Data availability: -
Code availability: -