| Expression pattern: |
UP |
| Associated gene: |
BCL2L11, Ago2 |
| Associated microRNA: |
miR-370-3p |
| Biological function: |
Promotes hypoxia-induced cardiomyocyte injury by inhibiting cell viability and proliferation and promoting apoptosis and inflammatory responses; silencing circ_0068655 mitigates these effects. |
| Molecular mechanism: |
circ_0068655 acts as a miR-370-3p sponge, relieving repression of BCL2L11 and regulating the caspase cascade to promote apoptosis and inflammation under hypoxia. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); inflammation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic target for intervening in myocardial injury following ischemia. |
| Description: |
hsa_circ_0068655 is up-regulated in hypoxia-treated human AC16 cardiomyocytes. It promotes hypoxia-induced cardiomyocyte injury by sponging miR-370-3p, thereby relieving repression of BCL2L11 and enhancing apoptosis and inflammatory responses while suppressing proliferation. Silencing circ_0068655 shows a protective effect in this in vitro myocardial ischemia model. |
| Confidence score: |
0.7626 |